Name : Abd El-Nasser Mohammed Abd El-Moneim Elashry
Date of Birth : March 15, 1971
Nationality : Egyptian
Position : Assistant research
Agricultural Genetic Engineering Research Institute (AGERI),
Agricultural Research Center (ARC), Cairo, Egypt
www.ageri.sci.eg/
Institute: www.ageri.sci.eg/topic5/mpp.htm
The members of AGERI: Click here!

Current Position: PhD student
Institut für Entwicklungsbiologie
Universität Göttingen, Germany
www.uni-goettingen.de

Education:

M. Sc., Nematology, October 1998, Faculty of Agriculture, University of Cairo, Egypt.
Finde the MA thesis here.
B. Sc., Plant Pathology , May 1992, Faculty of Agriculture, University of Cairo, Egypt.

Career and Employment History:

• May 2002: European C. elegans meeting - www.iigb.na.cnr.it/ewm/
• July 2001: Participant at 13th International C. elegans Meeting 2001, Los Angelos (USA) - www.arc.ucla.edu/celegans/
• Since July 2000: PhD research at University of Goettingen (Germany), Institute of Developmental Biology (Institut für Entwicklungsbiologie), topic: Gene function of some of the high mobility group proteins "HMG" in C. elegans
• Since October 1998: Assistant research at Agricultural Genetic Engineering Research Institute (AGERI).
• October 1994 until 1998 : Research assistant at Agricultural Genetic Engineering Research Institute (AGERI), Project: Molecular Characterization of root-knot nematodes (Meloidogyne Spp.) in Egypt
• June 1992 until October 1994: Master student in Genetic Engineering of Entomopathogenic Nematodes Project, National Agricultural Research Project (NARP).
  

Research experience:

1. Using different techniques with C.elegans for the determination of gene function:
   Immunostaining, flourescent proteins (GFP, YFP and CFP), and RNA interference (RNAi) by microinjection and feeding and visualizing the gene expression pattern by laser scanning microscope (LSM).
2. The data analysis of molecular markers by the Numerical Taxonomy System (NTSys) program to calculate the phylogenetic relationships.
3. Identification of root-knot nematodes (Meloidogyne spp) by using Random Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR), Restriction Fragment Length Polymorphism (RFLP), Pulsic Field Gel Electrophoresis (PFGE) karyotyping and southern blotting techniques.
4. Isolation and purification of Meloidogyne spp. and Heterodera spp. from soil as well as infected plants and rearing them in-vivo on their host and in-vitro.
5. Identification of Meloidogyne spp., and their races, by using differential hosts test and perineal patterns technique.
6. Isolation of entomopathogenic nematodes and culturing them on natural hosts and artificial media.
7. Isolation, rearing and using Pasturia penetrans as a biological control agent against Meloidogyne spp.
8. Studying the nematicidal effect and mode of action of the Bacillus thurengensis (BT) and its toxins on root-knot nematodes by studying the lethal effect of its crude extract and purified proteins based on in-vivo and in-vitro assays.
9. Using plant tissue culture and plant transformation techniques.
   

Scientific courses:

• Practical courses about molecular and developmental biology techniques:
  Drosophila, mouse, tissue cultures, human being, chicken, frog
  University of Goettingen (2001 - 2003).
• Rearing of root knot-nematodes (Meloidogyne spp.) and cyst nematodes (Heterodera spp.) in-vitro on root cultures, Kiel University, Germany, Dec. 1999.
• International training course on micropropagation and related techniques for the conservation and use of plant genetic resources and the improvement of crops, (August 12 to September 8, 1997), German Foundation for International Development, Germany.
• Crop Diseases and their control, Plant Pathology Institute, Agricultural Research Center (ARC), June 1994.

Computer courses:

• Bioinformatics course, University of Goettingen, Dec. 2002.
• SPSS : from Economic studies Center, Faculty of Agriculture, Cairo University, 23 March-13 April, 1996.
• Lotus 123 : from AUC, April 15- May 27, 1995.
• Word Perfect: from AUC, Jan. 22- Feb. 21, 1995.
• DBase IV: from AUC, Oct. 26- Nov. 21, 1994.

Languages courses:

• German: Grundstufe II (Elementary level II), VHS, 2002
• German: Grundstufe I (Elementary level), VHS, 2001
• English: upper intermediate: from American University of Cairo (AUC), summer 1995
• English: Intermediate level: from AUC, March 22- May 25, 1994
• English: Elementary B level course: from AUC, Jan. 18- March 17, 1994

References:

Dr. Ekkehard Schulze
Department of Developmental Biology
Zoology Institute, University of Goettingen

Prof . Magdy A. Madkour
Director, AGERI

Prof. Nagwa Abd El-Bary
Department of Zoology, Faculty of Agriculture, Cairo University.

Prof. Mohamed M. El-Saedy
Senior Scientist, AGERI